recO [2018-11-08 13:24:00]

mediator of RecA binding to ssDNA, required for the formation of RecA DNA repair centers, required for efficient survival and replication restart after replication-transcription conflicts

locus: BSU25280

pI: 8.21

mw: 29.20 kDa

protein length: 255 aa

gene length: 765 bp

function: DNA repair/ recombination

product: mediator of RecA binding to ssDNA

essential: no

synonyms: yqxN,yqfI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,608,946 → 2,609,713

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]

The protein

Catalyzed reaction/ biological activity

• provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] access to ssDNA during chromosomal transformation (together with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA]) [Pubmed|22373918]
• catalyzes annealing of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] or [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]/[protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation [Pubmed|22373918]
• required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair centers (together with [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]) [Pubmed|24891441]

Protein family

• recO family (according to Swiss-Prot)

Structure

• [PDB|2V1C] (the [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]-[protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] complex from ''Deinococcus radiodurans'', [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]: 52% identity, 78% similarity, [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO]: 30%/ 58%) [Pubmed|17581636]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)
• localizes to the DNA entry pole during transformation [Pubmed|22373918]

Expression and Regulation

Operons

• genes: [gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]-[gene|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era]-[gene|33FBC31E8C24177137B0BDD8B46C1AFFD7F7B038|yqzL]-[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|6330116], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|6330116], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• MGNA-C494 (yqxN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2492 NBRP B. subtilis, Japan]
• 1A892 ( ''recO''::''cat''), [Pubmed|10323239], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
• BKE25280 ( ''recO''::''ermC''), (available at the [http://www.bgsc.org/ BGSC] and in [SW|Fabian Commichau]'s lab) [pubmed|28189581]
• BP738 ( ''recO''::''tet''), (available in [SW|Fabian Commichau]'s lab)
• BP774 ( ''recO''::''ermC''), (available in [SW|Fabian Commichau]'s lab)
• BKE25280 (Δ[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
• BKK25280 (Δ[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT

References

Reviews

• 22933559

Original publications

• 24891441,18599486,19730681,20581116,22373918,17581636,21170359,24285298,24285298,25939832,26001966,15186413,10323239,26786319,23779106